Specificity of alpha-chymotrypsin with exposed carboxyl groups blocked.

Specificity of chymotrypsin B.

Brown, Shupe, and Laskowski (1) have described the isolation, from beef pancreas, of a crystalline proteolytic enzyme which they have named “activated protein B” or “chymotrypsin B.” Through the kindness of Dr. Laskowski who provided us with a 4 times recrystallized sample of this enzyme, it was possible to examine its action on a series of synthetic peptides and peptide derivatives. The data p...

Histochemical Demonstration of Protein-Bound Alpha-Acylamido Carboxyl Groups

A method has been developed to demonstrate the alpha-acylamido carboxyl groups of protein, taking advantage of the fact that acylamido carboxyl groups are converted to ketonic carbonyls by the action of acetic anhydride and absolute pyridine. The method utilizes deparaffinized sections of tissues fixed in a variety of fixatives. Following the conversion of carboxyls to the methyl ketones, the l...

Specific reactions of dinitrofluorobenzene with active groups of chymotrypsin.

The sulfhydryl, disulfide (l), phenolic (2), imidazole (3), and amino groups (4) of proteins exhibit varying degrees of reactivity in the native protein. Sorm and Rychlfk (5) have shown that the protease and milkclotting activities of chymotrypsin (ChTr) drop with increasing extent of substitution by 2,4dinitrofluorobenzene (DNFB, or Sanger’s reagent). The esterase activity of ChTr containing t...

Effectiveness of Alpha Chymotrypsin on the Treatment of Severe Paralysis due to Bungarus Multicinctus Snakebite

Cataract Extraction with Alpha-chymotrypsin a Controlled Trial.

First fifty intracapsular extractions were performed in an uncontrolled series so that the operator should become thoroughly familiar with the technique. In order to make a true assessment fifty ampoules were prepared by the hospital pharmacist with their accompanying ampoule of diluent, and these were numbered 1 to 50. Some of the ampoules contained active A.C.T. and the others an inert substa...